Journal: Advanced Science

Article Title: Arrayed Magnetic Accelerator Synergistically Enhances Orthodontic Tooth Movement via Microtubule Assembly‐Mediated Periodontal Remodeling

doi: 10.1002/advs.77447

Figure Lengend Snippet: AMOA promotes osteoclast and inflammatory remodeling in periodontal area. (a) Representative images of immunohistochemical staining for CTSK, TNF‐α, IL‐1β. CTSK staining at the periodontal ligament‑alveolar bone interface. Scale bar: 100 µm(CTSK), 20 µm(TNF‐α), and 20 µm(IL‐1β). (b) Statistics of numbers of CTSK positive cells in the alveolar bone and percentage of TNF‐α, IL‐1β positive cells per field in the periodontal ligament of the first molar. Mean ± SD. n = 4. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.001. (c) Diagram of the possible mechanism by which static magnetic field accelerates orthodontic tooth movement.

Article Snippet: Primary antibodies against rat CTSK (Proteintech, 11239‐1‐AP, 1:200), TNF‐α (Proteintech, 60291‐1‐Ig, 1:200) and IL‐1β (Proteintech, 26048‐1‐AP, 1:200) were used.

Techniques: Immunohistochemical staining, Staining

Journal: The Journal of Biological Chemistry

Article Title: Siah2 regulates lipid uptake in adipose tissue macrophages

doi: 10.1016/j.jbc.2026.111380

Figure Lengend Snippet: Lysosomal program is upregulated in ATMs independent of Siah2 . A , relative mRNA expression of lysosomal genes in fl/fl and Siah2 MacKO BMDMs and BM-ATMs. B , representative Western blot of lysosomal enzymes relative to β-actin in fl/fl and Siah2 MacKO BMDMs and BM-ATMs. The β-actin panel is the same as used in F . The blot shown in F was reprobed for the CTSK image in B . C , enzyme activity of CTSK and LAL in fl/fl and Siah2 MacKO BMDMs and BM-ATMs. D and E , relative mRNA expression of Atgl ( D ) and Hilpda ( E ) in fl/fl and Siah2 MacKO BMDMs and BM-ATMs. F , representative Western blot of ATGL and HILPDA relative to β-actin in fl/fl and Siah2 MacKO BMDMs and BM-ATMs. G , basal glycerol release in fl/fl and Siah2 MacKO BMDMs and BM-ATMs treated with vehicle or 50 μM Atglistatin or 0.1 μM Lalistat-1 for 24 h. H , glycerol release in fl/fl and Siah2 MacKO BM-ATMs treated with vehicle or 50 μM Atglistatin or 0.1 μM Lalistat-1 for 24 h with or without 0.5 mM IBMX (activated lipolysis). I and J , representative confocal images ( I ) and quantification ( J ) of fl/fl and Siah2 MacKO BM-ATMs stained for BODIPY 493/503 (LD, green ), LysoTracker (lysosomes, magenta ), and DAPI (nuclei, blue ) showing colocalization of LD and lysosomes. The scale bar represents 10 μm. Red signals of LysoTracker were converted to magenta using the LUT function in ImageJ. Statistics are reported as mean ± SD using an unpaired t test with Welch’s correction, and each dot denotes technical replicates representative of three to five independent experiments. A , D , E , G , H , and J , statistics are reported as mean ± SD using two-way ANOVA with Tukey’s multiple comparisons test ( C ). p Values are indicated on the graphs. ATGL, adipose triglyceride lipase; ATM, adipose tissue macrophage; BM-ATM, bone marrow–derived ATM; BMDM, bone marrow–derived macrophage; CTSK, cathepsin K; DAPI, 4′,6-diamidino-2-phenylindole; fl/fl, Siah2 flox/flox ; HILPDA, hypoxia-inducible LD-associated protein; IBMX, 3-isobutyl-1-methylxanthine; LAL, lysosomal acid lipase; LD, lipid droplet; MacKO, Siah2 MacKO ; Siah2, seven in absentia homolog 2.

Article Snippet: CTSK activity in BMDMs and BM-ATMs was measured using the Cathepsin K assay kit (Novus Biologicals, catalog no.: NBP2-54842) according to the manufacturer’s protocol.

Techniques: Expressing, Western Blot, Activity Assay, Staining, Derivative Assay